High Performance Liquid Chromatography Lab
One of the Natural Toxins Research Center’s main functions is to isolated components from venom and animal sera to be used in biomedical research. The center has a sophisticated collection of high performance liquid chromatography (HPLC) systems in which venom and animal sera are being separated and analyzed on a daily basis. There are numerous proteins found in snake venom, which makes the isolation of a single protein molecule a tedious and time consuming task. Having multiple HPLC systems allows the researcher to set up a series of columns having different fractionating capabilities. Separation of proteins can be done according to their physical properties such as their size, shape, charge, hydrophobicity, and their affinity for other molecules. The scientific terms for such separations are ion exchange (cation and anion), size exclusion, reverse phase, and affinity chromatography. The term HPLC was first used to describe the separation of molecules under high pressure in a stainless steel column filled with a matrix. In HPLC, both pump control and data acquisition can be done under the control a computer. One computer can control up to four HPLC systems or each system can stand-alone.Our aim is help the students obtain a visual understanding of HPLC as it relates to separation of proteins, as well as providing a starting point for HPLC purification procedures.
Tissue Culture Facility
Molecular Biology Facility
This page was last updated on: April 21, 2014