Texas A&M University-Kingsville

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NNTRC Laboratories

High Performance Liquid Chromatography Lab 

One of the Natural Toxins Research Center’s main functions is to isolated components from venom and animal sera to be used in biomedical research.  The center has a sophisticated collection of high performance liquid chromatography (HPLC) systems in which venom and animal sera are being separated and analyzed on a daily basis. There are numerous proteins found in snake venom, which makes the isolation of a single protein molecule a tedious and time consuming task.  Having multiple HPLC systems allows the researcher to set up a series of columns having different fractionating capabilities. Separation of proteins can be done according to their physical properties such as their size, shape, charge, hydrophobicity, and their affinity for other molecules.  The scientific terms for such separations are ion exchange (cation and anion), size exclusion, reverse phase, and affinity chromatography. The term HPLC was first used to describe the separation of molecules under high pressure in a stainless steel column filled with a matrix. In HPLC, both pump control and data acquisition can be done under the control a computer.  One computer can control up to four HPLC systems or each system can stand-alone.Our aim is help the students obtain a visual understanding of HPLC as it relates to separation of proteins, as well as providing a starting point for HPLC purification procedures.   Explanations of four types of HPLC separations can be found by clicking the links below.

Absorbence  Sample Settling

Imaging Center  Centrifuge Machinery

Lab Station  Storage

Two Absorbance towers

Tissue Culture Facility

Lab Station Utility Cabinet

Purification Hub  Centrifuge Station

Freezer

Molecular Biology Facility 

Work Table Sample Station

Centrifuge  Boiling Station

Mass Balance Sanitary Station

General Lab

Freezer Storage     Lab Station
Lab Table 

Hematology Lab

General Hematology  Agitator Station 
 Purification Station General Station 2
Coagulation Stations

This page was last updated on: March 26, 2014