NNTRC Research Assistant Professor

Personnel Profile

Dr. Montamas Suntravat, NNTRC Research Assistant Professor

Dr. Montamas Suntravat

Education

2010 - B.S., Medical Technology, Chulalongkorn University, Bangkok, Thailand

  • GPA: 4.00

  • Thesis project: “Effect of RVV-X and RVV-X-specific antivenom on renal functions and coagulopathy in rats”

  • Spent one year in the National Natural Toxins Research Center at Texas A&M University-Kingsville conducting scientific research on venom research, writing progress reports, attending weekly laboratory meetings, presenting seminars (February 2009-January 2010)

2001 - B.S., Medical Technology, Chulalongkorn University, Bangkok, Thailand

  • GPA: 3.53

Experience

Employment

2018 - Present       Assistant Professor, National Natural Toxins Research Center (NNTRC),

                              Department of Chemistry, Texas A&M University-Kingsville (TAMUK)

2015 - 2018           Research Assistant Professor, NNTRC, TAMUK

2011 - 2015           Research Scientist, NNTRC, TAMUK

2010 - 2011           Postdoctoral Fellow, Snake bite and Venom Research Unit, Faculty of Medicine,

                              Chulalongkorn University, Bangkok, Thailand      

2000 - 2009           Ph.D. Student Researcher, Snake bite and Venom Research Unit, Faculty of

                              Medicine, Chulalongkorn University, Thailand

Researcher

  • Conducting biomedical research in the National Natural Toxins Research Center at Texas A&M University-Kingsville (January 2011-present).
  • Snake bite and Venom Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand (January 2010-November 2010)
  • Visiting Scientist at the National Natural Toxins Research Center at Texas A&M University (February 2009-January 2010)
  • Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand (January 2006-June 2006)
  • Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand (September 2004-December 2005)

Certifications and Training:

  • American Medical Technologist (AMT) ID: 2739978
  • Attended Professional Grant Development Workshop at Texas A&M University-Kingsville (2011)
  • Mentor for undergraduate students in the Ronald E. McNair Scholars Summer Research program at Texas A&M University-Kingsville (2009)
  • Hazard Communication/Laboratory Safety Training Session, Texas A&M University-Kingsville, USA (2009)
  • Certified Medical Technologist, Thailand (2000)
  • Participated in a four-month Medical Technologist training program sponsored by Professional Laboratory Management Corp. CO. LTD., Bangkok, Thailand (2000)
  • Participated in a three-month Medical Technologist training program at Nopparat Rajathanee Hospital, Bangkok, Thailand (1998)

Laboratory Skills & Experience

  • Isolation of mRNA and DNA
  • Construction of cDNA library from Crotalus adamanteus snake venom gland
  • DNA cloning
  • Polymerase Chain Reaction
  • Protein expression, Protein purification by High performance liquid chromatography (HPLC) techniques
  • Mass spectrometry
  • Western blot analysis
  • ELISA
  • SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
  • Agarose gel electrophoresis
  • Antivenom production and purification
  • Numerous biological assays for screening venoms including anticoagulant and procoagulant assays
  • Platelet aggregation assays
  • Capillary electrophoresis
  • Electrophoretic titration
  • Histopathological techniques
  • Tissue culture techniques
  • Drawing blood
  • Working knowledge of dissection rat kidneys and renal hemodynamics and functions.

Honors and Professional Memberships

  • Member, American Association for Cancer Research (AACR) (2018-present)

  • Member, American Society for Microbiology (ASM) (2018-present)

  • Member, International Society on Toxinology (IST) (2012-present)

  • Member, Society of Toxicology (SOT) (2013)

  • First class honor for Ph.D. poster presentation at the eleventh Royal Golden Jubilee-Ph.D. Congress, Pattaya, Thailand (2010)

  • Recipient of Chulalongkorn University 90th Anniversary Fund, Thailand (2007-2008)

  • Recipient of Royal Golden Jubilee Ph.D. Scholarship, Bangkok, Thailand (2000-2005)

  • Academic honors award for the highest score in Immunology, Faculty of Allied Health Sciences, Chulalongkorn University, Thailand (2000)

  • Academic honors award for the highest score in Hematology, Faculty of Allied Health Sciences, Chulalongkorn University, Thailand (2000)

Lectures/ Presentations

Invited Lectures

  • Venom Week VI. The Acute Effects Of Snake Venom CRiSP Toxins On Blood And Lymphatic Endothelial Cell Permeability: New Insights Into The Pathophysiology Of Snakebite. Texas A&M University-Kingsville, TX, March 14-17, 2018.
  • The 19th European Section-International Society on Toxinology (EU-IST). The acute effects of snake venom CRiSP toxins on blood and lymphatic endothelial cell permeability: new insights into the pathophysiology of snakebite. Yerevan, Armenia, September, 22-26, 2018.
  • General Biology I (Honor), Texas A&M University-Kingsville, Kingsville, Texas, USA (September, 2011).
  • General Biology I, Texas A&M University-Kingsville, Kingsville, Texas, USA (September, 2011).
  • Microbiology and Parasitology for Nurses (International Program, English language), Christian University, Thailand (June 2010-September 2010).
  • Microbiology and Parasitology for Nurses, Christian University, Thailand (June 2010-September 2010).

Poster Presentations (English)

  • Montamas Suntravat, Issarang Nuchprayoon. Cross reactivity of two polyclonal antibodies specific to recombinant Russell’s viper venom-factor X activator (RVV-X) heavy chain and light chain against Cryptelytrops albolabris and Calloselasma rhodostoma venoms. The 14TH International Congress of Immunology. August 22-27, 2010. Kobe, Japan.
  • Montamas Suntravat, Mariem Yusuksawad, Issarang Nuchprayoon. Effect of Russell’s viper venom-factor X activator (RVV-X) from Daboia russellii siamensis venom on renal hemodynamics in Sprague-Dawley rats. The eleventh RGJ-Ph.D. Congress (RGJ-Ph.D. Congress XI). April 1-3, 2010. Pattaya, Thailand.
  • Montamas Suntravat, Issarang Nuchprayoon, Tracey Alvarado, Pérez JC. Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell’s viper venom-factor X (RVV-X) activator. Viper day. January 22, 2010. Texas A&M University-Kingsville, Texas, USA.
  • Montamas Suntravat, Issarang Nuchprayoon. Effect of Russell’s viper coagulation factor X activator (RVV-X) from Daboia russellii siamensis venom on coagulopathy in rats. The 8TH IST - ASIA PACIFIC Meeting on Animal, Plant and Microbial Toxins. December 2-6, 2008. Hanoi, Vietnam.
  • Montamas Suntravat, Issarang Nuchprayoon. Molecular cloning and expression of Factor X activator of Daboia russellii siamensis. The Fifth Princess Chulabhorn International Science Congress-Evolving genetics and its global impact. August 16-20, 2004. Bangkok, Thailand.

Publications

  1. Suntravat, M., Langlais PR, Sánchez EE, Nielsen VG (2018). CatroxMP-II: a heme-modulated fibrinogenolytic metalloproteinase isolated from Crotalus atrox Biometals. 14.
  2. Martin-Martin, I., Chagas, A.C., Guimaraes-Costa, A.B., Amo, L., Oliveira, F., Moore, I.N., DeSouza-Vieira, T.S., Sanchez, E.E., Suntravat, M., Valenzuela, J.G., Ribeiro, J.M.C., Calvo, E. (2018). Immunity to LuloHya and Lundep, the salivary spreading factors from Lutzomyia longipalpis, protects against Leishmania major infection. PLoS Pathog. 14: e1007006.
  3. Sánchez, E.E., González, R., Lucena, S., García, S., Finol, H.J., Suntravat, M., Girón, M.E., Fernández, I., Rodríguez-Acosta, A. (2018). Crotamine-like from Southern Pacific rattlesnake (Crotalus oreganus helleri) Venom acts on human leukemia (K-562) cell lines and produces ultrastructural changes on mice adrenal gland. Ultrastruct Pathol. 42: 116-123.
  4. Cantu Jr., M, Mallela, S., Nyguen, M., Baez, R., Parra, V., Johnson, R., Wilson, R., Suntravat, M., Lucena, S., Rodriguez-Acosta, A., Sanchez, E. (2017). The binding effectiveness of anti-r-disintegrin polyclonal antibodies against disintegrins and PII and PIII metalloproteases: An immunological survey of type A, B and A + B venoms from Mohave rattlesnakes. Comparative Biochemistry and Physiology, Part C. 191: 168-176.
  5. Rodríguez-Acosta, A., Lucena, S., Alfonso, A., Goins, A., Walls, R., Guerrero, B., Suntravat, M., Sánchez, E.E. (2016). Biological and biochemical characterization of venom from the broad-banded copperhead (Agkistrodon contortrix laticinctus): isolation of two new dimeric disintegrins. Anim Biol Leiden Neth. 66: 173-187.
  6. Suntravat, M., Helmke, T.J., Atphaisit, C., Cuevas, E., Lucena, S.E., Uzcátegui, N.L., Sánchez, E.E., Rodriguez-Acosta, A. (2016). Expression, purification, and analysis of three recombinant ECD disintegrins (r-colombistatins) from P-III class snake venom metalloproteinases affecting platelet aggregation and SK-MEL-28 cell adhesion. Toxicon. 122: 43-49.
  7. Komives, C.F., Sanchez, E.E., Rathore, A.S., White, B., Suntravat, M., Balderrama, M., Cifelli, A., Joshi, V. (2016). Opossum peptide that can neutralize rattlesnake venom is expressed in Escherichia coli. Biotechnol Prog. 7.
  8. Borja, M., Galan, J.A., Cantu, E.,  Zugasti-Cruz, A., Rodriguez-Acosta, A., Lazcano, D., Lucena, S., Suntravat, M., Sánchez, E.E. (2016). Morulustatin, a disintegrin that inhibits ADP-induced platelet aggregation, isolated from the Mexican Tamaulipan Rock Rattlesnake (Crotalus lepidus morulus). Revista Científica, FCV-LUZ 86-94.
  9. Suntravat, M., Uzcategui, N.L., Atphaisit, C., Helmke, T.J., Lucena, S.E., Sánchez, E.E., Rodríguez-Acosta, A. (2016). Erratum to: Gene expression profiling of the venom gland from the Venezuelan mapanare (Bothrops colombiensis) using expressed sequence tags (ESTs). BMC Mol Biol. 17: 13.
  10. Margres, M.J., Walls, R., Suntravat, M., Lucena, S., Sánchez, E.E., Rokyta, D.R. (2016). Functional characterizations of venom phenotypes in the eastern diamondback rattlesnake (Crotalus adamanteus) and evidence for expression-driven divergence in toxic activities among populations. Toxicon. 119: 28-38.
  11. Lucena, S., Rodríguez-Acosta, A., Grilli, E., Alfonso, A., Goins, A., Ogbata, I., Walls, R., Suntravat, M., Uzcátegui, N.L., Guerrero, B., Sánchez, E.E. (2016). The characterization of trans-pecos copperhead (Agkistrodon contortrix pictigaster) venom and isolation of two new dimeric disintegrins. Biologicals. 44: 191-197.
  12. Suntravat, M., Barret, H.S., Jurica, C.A., Lucena, S.E., Perez, J.C., Sánchez, E.E. (2015). Recombinant disintegrin (r-Cam-dis) from Crotalus adamanteus inhibits adhesion of human pancreatic cancer cell lines to laminin-1 and vitronectin. J Venom Res. 6: 1-10.
  13. Lucena, S.E., Castro, R., Lundin, C., Hofstetter, A., Alaniz, A., Suntravat, M., Sánchez, E.E. (2015). Inhibition of pancreatic tumoral cells by snake venom disintegrins. Toxicon. 93, 136-143.
  14. Lucena, S.E., Romo, K., Suntravat, M., Sánchez, E.E. (2014). Anti-angiogenic activities of two recombinant disintegrins derived from the Mohave and Prairie rattlesnakes. Toxicon. 78, 10-17.
  15. Suntravat, M., Jia, Y., Lucena, S.E., Sánchez, E.E., Pérez, J.C. (2013). cDNA cloning of a novel snake venom metalloproteinase from eastern diamondback rattlesnake (Crotalus adamanteus), and expression of its disintegrin domain with anti-platelet effects. Toxicon. 64: 43-54.
  16. Khunsap, S., Pakmanee, N., Khow, O., Chanhome, L., Sitprija, V., Suntravat, M., Lucena, S.E., Pérez, J.C., Sánchez, E.E. (2011). Purification of a phospholipase A2 from Daboia russelii siamensis venom with anticancer effects. J Venom Res. 2: 42-51.
  17. Suntravat, M., Yusuksawad, M., Sereemaspun, A., Pérez, J.C., Nuchprayoon, I. (2011). Effect of Russell’s viper venom-factor X activator (RVV-X) on DIC, renal hemodynamics and function, and histopathology. Toxicon. 58(3): 230-238.
  18. Suntravat, M., Nuchprayoon,  I. (2011). Recombinant Russell’s viper venom-factor X activator (RVV-X)-specific antibody: neutralization and cross-reactivity with Cryptelytrops albolabris and Calloselasma rhodostoma venoms. Asain Biomedicine. 5(3): 371-379.
  19. Suntravat, M., Nuchprayoon, I., Pérez, J.C. (2010). Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell’s viper venom-factor X (RVV-X) activator. Toxicon. 15; 56(4): 544-553.
  20. Nuchprayoon, S., Sanprasert, V., Suntravat, M., Kraivichian, K., Saksirisampant, W., Nuchprayoon, I. (2003). Study of specific IgG subclass antibodies for diagnosis of Gnathostoma spinigerum. Parasitol Res. 91(2): 137-143.

Research and Creative Activities

Ongoing Research Support

  • 1R15HL137134-01 (PI: M. Suntravat)           04/01/2017- 03/31/2020

NIH/NHLBI

“The acute effects of snake venom CRiSP toxins on blood and lymphatic endothelial cell permeability: new insights into the pathophysiology of snakebite”

This is an NIH Academic Research Enhancement Award (AREA) Program (R15) to support the research projects to expose undergraduate and graduate students to research, and to strengthen the research environment of the National Natural Toxins Research Center (NNTRC) at Texas A&M University – Kingsville. Funding from this award supports studies directed toward the characterization of the effects of a specific family of snake venom toxins, the svCRiSPs (snake venom Cysteine-Rich Secretory Proteins), on the function of blood and lymphatic endothelial cells that will provide new insights into the pathophysiology of snakebite.

$412,735

  • 5 P40 OD010960 (PI: E. Sanchez)            05/01/2014 - 04/30/2019

NIH/NCATS

“Viper Resource Center at Texas A&M University - Kingsville (TAMUK)”

This is an NIH Animal and Biological Material Resource Center Grant (P40) to support the operation of the National Natural Toxin Research Center (NNTRC) at Texas A&M University – Kingsville. Funding from this award supports the operation of the Perez Serpentarium, the collection and distribution of snake venoms and snake venom-derived reagents to academic and commercial researchers world-wide. The award also supports studies directed toward the characterization of the biological activity of certain snake venom toxins.

$2,984,946

Completed Research Support

  • Suntravat (PI)                 01/29/2018-07/16/2018

TAMUK Council for Undergraduate Research (TCUR), Texas A&M University-Kingsville

“Antibacterial activity of 22 snake venoms from families Viperidae and Elapidae”

$4,980

  • Suntravat (PI)                 09/01/2012-12/01/2012

Cisne Enterprises, Inc., Odessa, TX

“Neutralization of local hemorrhagic activity and lethality of Western Diamondback Rattlesnake (Crotalus atrox) venom with silicon dioxide (LIPH)”

$19,121

  • Suntravat (PI)                09/01/2012-08/31/2013

University Research Awards, Texas A&M University-Kingsville

“Study a Novel Venom Recombinant Disintegrin for Anti-Pancreatic Cancer”

$14,960

  • Suntravat (PI)               10/15/2011-05/01/2012

TAMUK Council for Undergraduate Research (TCUR), Texas A&M University-Kingsville

“Cloning, expression, and characterization of recombinant novel RGD-disintegrin and its shorter peptides from Crotalus adamanteus”

$1,000

  • Suntravat (PI)               09/01/2010-08/31/2011

Research and Scholarly Activity Awards program, College of Arts and Sciences, TAMUK

“Purification, cloning, and expression of disintegrins from Crotalus adamanteus cDNA libraries”

$1,500

Grants

  • Grant from the College of Art and Sciences Faculty Research and Scholarly Activity Awards program, Texas A&M University-Kingsville for the research project: “Purification, cloning, and expression of disintegrins from Crotalus adamanteus cDNA libraries” (2010)

Contact Information

National Natural Toxins Research Center
975 W. Avenue B, Kleberg Hall Rm#105
MSC Texas A&M University-Kingsville
Kingsville, Texas 78363-8202
voice: (361) 593-3794
fax:
email: montamas.suntravat@tamuk.edu
Personal Website:

This page was last updated on: February 7, 2019